Generator

Part:BBa_K2014007

Designed by: Daria Niewiadomska   Group: iGEM16_UAM_Poznan   (2016-10-12)
Revision as of 08:52, 12 October 2016 by Registry (Talk | contribs)

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AraC-pBAD->sfGFP_8R


We were using standard AraC-pBAD promoter to compare the influence of different ORFs structures coding for the same protein sfGFP and also the stability of the mRNA during translation.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961
    Illegal SapI.rc site found at 1260


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Parameters
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