Coding
Part:BBa_K1949030:Design
Designed by: Yoshio Takata Group: iGEM16_Tokyo_Tech (2016-10-06)
yafO
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Unknown
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Unknown
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
yafO gene fragment amplified by PCR contains XbaI site at N-terminal and SpeI and PstI site at C-terminal. Both terminals were cut by respective restriction enzymes and the fragment used for ligation with iGEM plasmid vectors.
fwd:5’-AAATCTAGATGCGGGTATTCAAAACAAAACTTA-3’
rev:5’-AAACTGCAGCGGCCGCTACTAGTATTATTAAAAACGCATGCGAAACGCTTCTGCCA-3’
Source
This part is derived from E.coli,BM26, amplified by using PCR.
===References===