Part:BBa_K1949030
yafO
Toxin-antitoxin (TA) systems are cassettes consisting of stable protein, toxin which function as mRNA interferase and unstable protein, antitoxin which destroys function of toxin. Many TA systems exist in the E. coli genome. They can inhibit cell growth or induce cell killing. YafO is one of the toxin and is co-expressed with YafN.
YafO is mRNA interferase, that is, it cleaves mRNA by endonuclease activity and inhibits protein synthesis. It is thought that YafO endonuclease activity is induced by binding to 50S subunit in 70S ribosome. Yafo is a rebosome-dependent mRNA interferase and cleaves coding regions of mRNAs.
It is also considered that YafO inhibits translation in two steps. Initially, the binding of the toxin to 70S ribosomes inhibits translation reversibly via binding with its cognate antitoxin. However, in the second step, as the toxin binding to ribosomes is prolonged, the latent ribonuclease activity of the toxin is induced to cleave mRNAs, which results in irreversible inhibition of protein synthesis.
Characterization
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 335
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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