Coding

Part:BBa_K2105000

Designed by: Nicholas Emery   Group: iGEM16_Michigan   (2016-10-11)
Revision as of 02:29, 12 October 2016 by Registry (Talk | contribs)

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LacZ delta M15 mutation

The lacZ delta M15 mutation encodes a form of beta-galactosidase lacking residues 11-41. This mutation causes it to be inactive until complemented by the lacZ alpha fragment. The lacZ delta M15 mutant is constitutively expressed in DH5 alpha cells. When the delta M15 mutant and the alpha fragment undergo alpha complementation, they are able to cleave the substrate X-gal to produce a blue product.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None