Generator

Part:BBa_K2014000

Designed by: Przemyslaw Nuc   Group: iGEM16_UAM_Poznan   (2016-10-11)
Revision as of 23:00, 11 October 2016 by Registry (Talk | contribs)

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pBAD-E15'UTR->sfGFP

In commercially available expression vectors AraC-pBAD promoter is used. We have found that pBAD without the AraC ORF is as efficient as AraC-pBAD and functional as well. The pBAD promoter can be induced with 0,2% arabinose. We have tested 3 5'UTR variants different from its original 5'UTR, and we have found that E1 5'UTR enhanced the gene expression level upon induction with arabinose.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 236
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 71
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 53
    Illegal SapI.rc site found at 365


[edit]
Categories
//awards/part_collection/2016
Parameters
None