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Generator
Part:BBa_K2014000
Designed by: Przemyslaw Nuc Group: iGEM16_UAM_Poznan (2016-10-11)
pBAD-E15'UTR->sfGFP
In commercially available expression vectors AraC-pBAD promoter is used. We have found that pBAD without the AraC ORF is as efficient as AraC-pBAD and functional as well. The pBAD promoter can be induced with 0,2% arabinose. We have tested 3 5'UTR variants different from its original 5'UTR, and we have found that E1 5'UTR enhanced the gene expression level upon induction with arabinose.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 236
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 71
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 53
Illegal SapI.rc site found at 365
[edit]
Categories
Parameters
//awards/part_collection/2016
None |