Coding

Part:BBa_K1949050:Design

Designed by: Yoshio Takata   Group: iGEM16_Tokyo_Tech   (2016-10-06)
Revision as of 19:09, 11 October 2016 by Kazuki (Talk | contribs)


amiE


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

A point mutation is introduced to amiE to remove the EcoRI recognition site; the 291th nucleotide was changed from A to a G.


Source

The DNA fragment of amiE was artificially synthesized.


References