Composite

Part:BBa_K1897021

Designed by: Keshiniy Madivannan   Group: iGEM16_NUS_Singapore   (2016-10-11)
Revision as of 16:11, 11 October 2016 by Registry (Talk | contribs)

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p70-33-ALR-Terminator

The construct contains one of the synthetic lldR promoters designed by team ETH-Zurich, IGEM 2015 (BBa_K1847008) with a weaker RBS (BBa_B0033), followed by a CDS of ALR (BBa_K1172901) and a lambda t0 terminator.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 78
    Illegal NheI site found at 101
    Illegal NheI site found at 499
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 423
    Illegal BamHI site found at 1125
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 541
    Illegal AgeI site found at 841
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 298


[edit]
Categories
Parameters
None