Composite

Part:BBa_K1961006

Designed by: Yi-Ting Lin   Group: iGEM16_HSiTAIWAN   (2016-10-11)
Revision as of 14:44, 11 October 2016 by Registry (Talk | contribs)

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Lead ion detector

This part is designed to detect lead ion following the concept of B. It consists of two parts: (1) PbrR generation:this part produces PbrR which can form a dimer of Pb2+/PbrR in the presence of Pb2+. (2) RFP expression: this part is controlled by a promoter(BBa_I721001) which has a binding site of dimer of Pb2+/PbrR. RFP expression can be observed when the dimer binds onto the promoter.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1281
    Illegal AgeI site found at 1393
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 686


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Categories
Parameters
None