Coding
Part:BBa_K1962007
Designed by: Frank Sargent Group: iGEM16_Dundee (2016-10-11)
Colicin E9::Ssp1 Chimera
This part contains an translational fusion protein between the truncated DNase-domain-lacking version of Colicin E9 (BBa_K1962006) and an antibacterial toxin (peptidoglycan hydrolase) called Ssp1 from Serratia marcescens. Ssp1 is an antibacterial effector normally secreted by the Type VI secretion system (T6SS) by Serratia marcescens and it has peptidoglycan endopeptidase activity.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1879
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1357
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1543
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |