Part:BBa_K1920001
Pl-RBS-RFP-TT
Glucose detection device, which uses red fluorescence protein E1010 as a Reporter . PI promoter consists of overlapping consensus CRP-binding sites and consensus RNA polymerase binding site. Therefore, the steric hindrance between CRP and RNA polymerase will cause downstream genes to be repressed at a high concentration of CRP. In E.coli strains( e.g.DH5α and Bl21), a high concentration of CRP is presented when the glucose concentration is low, because, with the increasing adenylyl cyclase activity, the cAMP increases. cAMP will bind the cAMP receptor protein (CRP) which, in its bound form, can specifically bind the consensus CRP-binding site of Pl promoter , leading to repression of downstream genes. In contrast, when the glucose concentration is high, the adenylyl cyclase activity, cAMP, and CRP are decreased in the bound form . Consequently, an increased expression of downstream RFP E1010 can be a reporter gene as well as an indicator of the presence of glucose can be.
This part works and is experimentally validated by NCKU_Tainan_2016 , further details are documented in experience page.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 617
Illegal AgeI site found at 729 - 1000COMPATIBLE WITH RFC[1000]
| None |