Composite

Part:BBa_K2088016

Designed by: Xia Chen   Group: iGEM16_NAU-CHINA   (2016-10-09)
Revision as of 10:45, 11 October 2016 by Malindaping (Talk | contribs)


nativeRBS-pbaA1A2B with a double Terminator

This part includes optimized mhbI (using Codon Optimization Tool of IDT) and a designed rbs (using RBS Calculator). We choose Escherichia coli K12 as the organism to optimize the sequence.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 2648
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 73
    Illegal NgoMIV site found at 116
    Illegal NgoMIV site found at 776
    Illegal NgoMIV site found at 1383
    Illegal NgoMIV site found at 1407
    Illegal AgeI site found at 1291
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None