Regulatory
pPCK1

Part:BBa_K2030001

Designed by: John Hellgren   Group: iGEM16_Chalmers_Gothenburg   (2016-08-02)
Revision as of 09:44, 11 October 2016 by Jannie (Talk | contribs)


pPCK1 S. cerevisiae promoter

The upstream regulatory sequence to the gene PCK1, coding for Phosphoenolpyruvate carboxykinase. This promoter is induced by glycerol, acetate and ethanol (10x) [1].

After sequencing of this part, it can be noted that the sequence differs in 3 positions when comparing against the reference genome (S228c). However, it matches perfectly with the genomic sequence of [http://www.yeastgenome.org/contig/JRIV01000108.1/overview CEN.PK2], where the promoter was amplified from.


Characterization

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 221
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 364
  • 1000
    COMPATIBLE WITH RFC[1000]


References

[1] K. Weinhandl, M. Winkler, A. Glieder, and A. Camattari, “Carbon source dependent promoters in yeasts,” Microbial Cell Factories, vol. 13, no. 1, 2014.

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