Coding

Part:BBa_K1916002

Designed by: Jonathan Chen   Group: iGEM16_UC_Davis   (2016-10-10)
Revision as of 03:31, 11 October 2016 by Registry (Talk | contribs)

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Cyan7427_1390g3 + intein-CBD tag

A GAF domain of the cyanobacteriochrome (CBCR) Cyan7427_1390 with an intein/chitin-binding domain attached to the C-terminus for purification. As with all CBCRs, NpF requires a phycocyanobilin co-factor, and must therefore be expressed in a system with heme oxygenase (BBa_I15008) and PcyA (BBa_I15009).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 576
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1096
    Illegal AgeI site found at 1186
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None