Part:BBa_K1926001

A cyclic promoter of Cyclin E from human genome

This part is a cyclic promoter of protein Cyclin E from human genome. It can lead to transcription of the downstream DNA sequence once in every G1 phase[1,2]

     1.	Hwang, H.C. and B.E. Clurman, Cyclin E in normal and neoplastic cell cycles. Oncogene, 2005. 24(17): p. 2776-86.

     2.	Ohtani, K., J. DeGregori, and J.R. Nevins, Regulation of the cyclin E gene by transcription factor E2F1. Proc Natl Acad Sci U S A, 1995. 92(26): p. 12146-50.

You may use this part to:

1) Express something in mammal cell lines particularly in G1 phases or once in every cell cycle by stable transfecting it into cell line;

2) Use it as a human promoter by transient transfecting it into cells.

Source:

The sequence was retrieved from Addgene. We got it from human genome through PCR using the following primers:

pCCNE-F: CGTGTTTACATTCCACCCGCGCCA

pCCNE-R: TGATGGGGCTGCTCCGGCCT

(Product: 986)

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NotI site found at 382
  Illegal NotI site found at 497
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 529
  Illegal XhoI site found at 1050
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 273
  Illegal NgoMIV site found at 313
  Illegal NgoMIV site found at 491
  
• 1000
  COMPATIBLE WITH RFC[1000]