Composite

Part:BBa_K1897018:Design

Designed by: Ang Shi Hui   Group: iGEM16_NUS_Singapore   (2016-10-10)
Revision as of 19:41, 10 October 2016 by Registry (Talk | contribs)

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LuxR + LuxI + Invasin


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1852
    Illegal BamHI site found at 1872
    Illegal XhoI site found at 4659
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 50
    Illegal AgeI site found at 1065
    Illegal AgeI site found at 2844
    Illegal AgeI site found at 4353
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

There is one HA tag available for characterisation of the LuxR and LuxI proteins produced via western blot, and two HA tag for Invasin protein.


Source

LuxR and LuxI are derived from Vibrio fischeri, while Invasin is derived from Yersinia pseudotuberculosis, from the coding sequence [Genbank M17448.1] (see BBa_K1897010.

References