Composite
Part:BBa_K1897018:Design
Designed by: Ang Shi Hui Group: iGEM16_NUS_Singapore (2016-10-10)
LuxR + LuxI + Invasin
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1852
Illegal BamHI site found at 1872
Illegal XhoI site found at 4659 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 50
Illegal AgeI site found at 1065
Illegal AgeI site found at 2844
Illegal AgeI site found at 4353 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
There is one HA tag available for characterisation of the LuxR and LuxI proteins produced via western blot, and two HA tag for Invasin protein.
Source
LuxR and LuxI are derived from Vibrio fischeri, while Invasin is derived from Yersinia pseudotuberculosis, from the coding sequence [Genbank M17448.1] (see BBa_K1897010.