Composite

Part:BBa_K1897011:Design

Designed by: Ang Shi Hui   Group: iGEM16_NUS_Singapore   (2016-10-10)
Revision as of 18:55, 10 October 2016 by Shihuiangle (Talk | contribs) (→‎Design Notes)


Invasin


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 4
    Illegal XhoI site found at 2791
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 976
    Illegal AgeI site found at 2485
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

There are two HA tags available for characterisation of the protein produced via western blot. Note that the stop codon for Invasin CDS BBa_K1897010 is shifted to after the HA tags.

Source

Invasin is derived from Yersinia pseudotuberculosis, from the coding sequence [Genbank M17448.1] (see No part name specified with partinfo tag.. The promoter was synthesized based on the sequence obtained from BBa_R0062. The ribosome binding site (RBS) was synthesized based on the sequence obtained from BBa_B0030. The transcription terminators were synthesized based on the sequence obtained from BBa_B0015.

References