Composite
Part:BBa_K1897011:Design
Designed by: Ang Shi Hui Group: iGEM16_NUS_Singapore (2016-10-10)
Invasin
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 4
Illegal XhoI site found at 2791 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 976
Illegal AgeI site found at 2485 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
There is one HA tag available for characterisation of the protein produced via western blot. Note that the stop codon for Invasin CDS BBa_K1897010 is shifted to after the HA tag.
Source
Invasin is derived from Yersinia pseudotuberculosis, from the coding sequence [Genbank M17448.1] (see No part name specified with partinfo tag.. The promoter was synthesized based on the sequence obtained from BBa_R0062. The ribosome binding site (RBS) was synthesized based on the sequence obtained from BBa_B0030. The transcription terminators were synthesized based on the sequence obtained from BBa_B0015.