Composite

Part:BBa_K1898550:Design

Designed by: Fiona Tsai   Group: iGEM16_TAS_Taipei   (2016-10-10)
Revision as of 15:28, 10 October 2016 by Registry (Talk | contribs)

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Strong promoter + Strong RBS + GFP + 10x Histidine tag + double terminator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 705


Design Notes

We made sure that there was no Ecor1, Xbal, Spel1, and Pst1 cutting sites in the DNA. The double stop codon at the end of BBa_E0040 was also removed.


Source

This construct was designed and sent to IDT for oligo synthesis.

References