Coding

Part:BBa_K1898400:Design

Designed by: Fiona Tsai   Group: iGEM16_TAS_Taipei   (2016-10-10)
Revision as of 15:16, 10 October 2016 by Registry (Talk | contribs)

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CRYAB, crystallin alpha B


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 26
    Illegal BamHI site found at 368
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 453
    Illegal SapI.rc site found at 313


Design Notes

We made sure that there was no Ecor1, Xbal, Spel1, and Pst1 cutting sites in the DNA. Primers were designed to remove the stop codon and were synthesized by Tri-I biotech.



Source

the cDNA or cryab is ordered from OriGene.

References