Part:BBa_K1933001

INPNC fused to 6xHis tag

INPNC codes N- and C- terminal domain of Ice-Nucleation Protein from Pseudomonas syringae, connected by a 6×His tag to be easily identified by Western blotting. [1]This is used as an anchoring motif for the cell surface display of passengers. This domain do not have signal peptide for anchoring on the cell surface[2] Surface displayed passengers retains enzyme activity even when it is linked to INPNC with 6× His tag. For more information, please visit [http://2016.igem.org/Team:Kyoto our wiki]

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 952
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 72
  Illegal NgoMIV site found at 405
  Illegal AgeI site found at 823
  
• 1000
  COMPATIBLE WITH RFC[1000]