Coding

Part:BBa_K1898100:Design

Designed by: Fiona Tsai   Group: iGEM16_TAS_Taipei   (2016-10-10)
Revision as of 14:47, 10 October 2016 by Fionat17112752 (Talk | contribs)


ch25h, cholesterol 25 hydroxylase


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We removed one point mutation from the cDNA with mutagenesis done by MissionBiotech. Primers were designed to remove the stop codon and were synthesized by Tri-I biotech.


Source

cDNA of ch25h was ordered from Sino BIological Inc

References