Composite

Part:BBa_K1898150:Design

Designed by: Fiona Tsai   Group: iGEM16_TAS_Taipei   (2016-10-10)
Revision as of 14:34, 10 October 2016 by Registry (Talk | contribs)

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Strong promoter + Strong RBS + CH25H + 10x His tag + Double terminator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We made sure that there was no Ecor1, Xbal, Spel1, and Pst1 cutting sites in the DNA.


Source

this DNA is synthesized by IDT.

References