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Part:BBa_I716002:Design

Designed by: Arthur Yu   Group: iGEM07_Berkeley_UC   (2007-06-18)
Revision as of 19:36, 18 June 2007 by Ayu (Talk | contribs) (→‎Design Notes)

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yfbE iron promoter basic part


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 25


Design Notes


This part is built in Biobrick version 2.0. In this format, parts are flanked by BglII and BamHI restriction sites. 

Bbrick yfbE promoter
[pcr] yfbE-F/R on E.coli MG1665 gen.     (437bp, BglII/XhoI)
[sub] into pBca9145-Bca1144#5            (BglII/XhoI, 2063+910, L)
[prod] pBca9145-yfbE (I716002)
---
AY005.yfbE-F   cgacaAGATCTatgatatatttttttgacttc
AY006.yfbE-R   cttatCTCGAGttaGGATCCcgagttcctccacgcccattg

Source

E. coli MG1665

References