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Part:BBa_I716002:Design

Designed by: Arthur Yu   Group: iGEM07_Berkeley_UC   (2007-06-18)
Revision as of 19:34, 18 June 2007 by Registry (Talk | contribs)

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yfbE iron promoter basic part


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 25


Design Notes

Bbrick yfbE promoter [pcr] yfbE-F/R on E.coli MG1665 gen. (437bp, BglII/XhoI) [sub] into pBca9145-Bca1144#5 (BglII/XhoI, 2063+910, L) [prod] pBca9145-yfbE (I716002) --- AY005.yfbE-F cgacaAGATCTatgatatatttttttgacttc AY006.yfbE-R cttatCTCGAGttaGGATCCcgagttcctccacgcccattg


Source

E. coli MG1665

References