Coding
Part:BBa_K1898110:Design
Designed by: Fiona Tsai Group: iGEM16_TAS_Taipei (2016-10-10)
ch25h, cholesterol 25 hydroxylase
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
We removed one point mutation that came with the cDNA on the 308 base pair with mutagenesis sent to MissionBiotech
Source
cDNA of ch25h was ordered from Sino BIological Inc