Part:BBa_K1933001

INPNC fused to 6xHis tag

INPNC codes N- and C- terminal domain of Ice-Nucleation Protein from Pseudomonas syringae, connected by a 6×His tag to be easily identified by Western blotting. [1]This is used as an anchoring motif for the cell surface display of passengers. This domain do not have signal peptide for anchoring on the cell surface[2] Surface displayed passengers retains enzyme activity even when it is linked to INPNC with 6× His tag. For more information, please visit our <a ref=http://2016.igem.org/Team:Kyoto> Wiki page</a>.