Device

Part:BBa_K1920002

Designed by: Chia-En Wong   Group: iGEM16_NCKU_Tainan   (2016-09-03)
Revision as of 10:43, 10 October 2016 by CalvinWong (Talk | contribs)

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PoPS->lysis

The lysis gene is downstream to constitutive promoter BBa_J23101 and ribosome binding site BBa_B0031 and encodes for the lysis protein in colicin-producing strains of bacteria. Once activated, it causes the host cell to lyse. It also removes the immunity protein out of colicin, and hence, activates the endonuclease activity of the colicin.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None