Composite

Part:BBa_K1926022

Designed by: Xinyi Liu   Group: iGEM16_SYSU-CHINA   (2016-10-10)
Revision as of 05:06, 10 October 2016 by CindyLiu (Talk | contribs)

This part is the SNAP-mCHERRY UNIT of cyclebow including a SNAP UNIT and a mCHERRY UNIT. The SNAP UNIT, which can be cut off by CRE, includes an sv40 nuclear location sequence (NLS), a covalent labeling of fusion proteins named SNAP-tag® and an sv40 terminator flanked by homodromous recognition site of recombinase CRE named loxP. The mCHERRY UNIT, which can be cut off by VIKA, includes a degradation tag named DBOX, a fluorescent gene mCherry and an sv40 terminator flanked by homodromous recognition site of recombinase VIKA named Vox.


You may use this part to:

1) Test the cut-off efficiency of recombinase CRE and VIKA;

2) Estimate the time needed for genome repair because mCherry can only be expressed after the cut off of SNAP UNIT and genome repair;

3) Fast cut off this part by transient transfecting recombinase gene into nucleus;

4) When there is no CRE, you can use this part as the SNAP UNIT, which can help localize proteins in vivo with BLOCK and dye from NEB company.

[edit]
Categories
//cds/reporter/rfp
//chassis/eukaryote/human
Parameters
None