Composite

Part:BBa_K1926012

Designed by: Xinyi Liu   Group: iGEM16_SYSU-CHINA   (2016-10-09)
Revision as of 04:57, 10 October 2016 by Registry (Talk | contribs)

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The VIKA UNIT: recombinase VIKA flanked by Vox

This part is the VIKA UNIT of cyclebow system including an sv40 nuclear location sequence (NLS), a recombinase gene named VIKA and an sv40 terminator flanked by homodromous recognition site of recombinase VIKA named Vox. The whole part can be cut off by VIKA. This part is the improvement of the BBa_K1641003，which is a plain VIKA.

You may use this part to:

1) Test the cut-off efficiency of recombinase VIKA by qRT-PCR;

2) Fast cut off this part by transient transfecting recombinase gene into nucleus;

3) Do lineage tracking.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//cds/enzyme/chromatinremodeling
//chassis/eukaryote/human
//function/recombination
Parameters
None