Project

Part:BBa_K1926003:Design

Designed by: Xinyi Liu   Group: iGEM16_SYSU-CHINA   (2016-10-09)
Revision as of 04:43, 10 October 2016 by Registry (Talk | contribs)

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A cyclic promoter of CDK4 from human genome


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 522
    Illegal NheI site found at 526
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 531
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 142
    Illegal AgeI site found at 173
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

None


Source

The sequence was retrieved from Addgene. We got it from human genome through PCR using the following primers: pCDK4-F: TGGTGGAGCGAAAAGGTGACAGCATC pCDK4-R: GCGGAAACTGGGAGGGCGGGGCGAA (Product: 457)


References