Project
Part:BBa_K1926002:Design
Designed by: Xinyi Liu Group: iGEM16_SYSU-CHINA (2016-10-09)
A cyclic promoter of Ki-67 from human genome
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 425
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 422
Design Notes
Mutation to remove Pst1
Source
The sequence was retrieved from Addgene. We got it from human genome through PCR using the following primers: pKi67-F: ACCTCTGCCCTCCGCCAGCCG pKi67-R: ACCCGGTGGCCCTACAGGCTACG (Product: 360)