Coding

Part:BBa_K1916000:Design

Designed by: Jonathan Chen   Group: iGEM16_UC_Davis   (2016-10-09)
Revision as of 04:26, 10 October 2016 by Registry (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)


NpF2164g5 + intein-CBD tag


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 531
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 531
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 531
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 531
    Illegal NgoMIV site found at 1027
    Illegal AgeI site found at 1117
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

It is not advisable to purify this protein using metal ion affinity columns. As such the fusion protein contains an intein-CBD tag and must be purified using chitin columns.


Source

From cyanobacteria genome

References