Coding

Part:BBa_K2114014:Design

Designed by: Wladislaw Stroukov   Group: iGEM16_Freiburg   (2016-10-09)
Revision as of 01:16, 10 October 2016 by WladStr (Talk | contribs)

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GST_HA_aHelix_CotG


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 688
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 85


Design Notes

Introduction of HA epitope tag and alpha helical linker by primer extensions and PCR.


Source

The cotB gene comes from the genome of B. subtilis. The anti-GFP nanobody is derived from camelid heavy-chain antibodies described by Kubala, M. H., Kovtun, O., Alexandrov, K. & Collins, B. M. Structural and thermodynamic analysis of the GFP:GFP-nanobody complex. Protein Sci. 19, 2389–2401 (2010).

References