Regulatory

Part:BBa_K2148003

Designed by: Chern Juin Ong   Group: iGEM16_Cambridge-JIC   (2016-10-09)
Revision as of 23:28, 9 October 2016 by Cjong (Talk | contribs) (→‎Usage and Biology)

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atpA promoter/5UTR

A level 0 Phytobrick coded as promoter/5UTR which carries the atpA promoter for expression of genes in Chlamydomonas reinhardtii.


Usage and Biology

This part can be used along with other Level 0 part to create a composite part which is a transcriptional unit with the desired Coding DNA Sequence (CDS).


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 543
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Characterisation

References

Drapier D, Suzuki H, Levy H, et al. The Chloroplast atpA Gene Cluster in Chlamydomonas reinhardtii  : Functional Analysis of a Polycistronic Transcription Unit. Plant Physiology. 1998;117(2):629-641.

Wannathong T, Waterhouse JC, Young REB, Economou CK, Purton S. New tools for chloroplast genetic engineering allow the synthesis of human growth hormone in the green alga Chlamydomonas reinhardtii. Applied Microbiology and Biotechnology. 2016;100:5467-5477. doi:10.1007/s00253-016-7354-6.

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Parameters
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