Regulatory

Part:BBa_K2148000

Designed by: Chern Juin Ong, Ciara McCarthy   Group: iGEM16_Cambridge-JIC   (2016-08-12)
Revision as of 22:51, 9 October 2016 by Cjong (Talk | contribs) (→‎Characterisation)


psaA1 promoter + 5

Promoter and 5'UTR sequence for the psaA gene in Chlamydomonas reinhardtii chloroplast, flanked with the Phytobrick fusion sites for a promoter with 5'UTR part. Submitted in the universal acceptor vector (psB1C3), and can be extracted using Bsa1 to give the necessary Phytobrick fusion site overhangs for Golden Gate assembly to produce a transcriptional unit with other Phytobricks.


Usage and Biology

This part can be used along with other Level 0 part to create a composite part which is a transcriptional unit with the desired Coding DNA Sequence (CDS).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Characterisation

References

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Categories
Parameters
None