Regulatory
Part:BBa_K1968001
Designed by: Paolo Marangio Group: iGEM16_Edinburgh_OG (2016-10-06)
Synechocystis Pcpc560 Phytobrick promoter
Pcpc560 stands for the 560bp region found upstream of the cpcB gene encoding the beta subunit of c-phycocyanin in Synechocystis sp. strain PCC 6803. This region has been reported to act as a promoter that is able of producing heterologous proteins at a level up to 15% of total soluble protein in Synechocystis, which is comparable to levels achieved in E. coli (Zhou et al., 2014). In light of this, this genomic region has been termed as the Pcpc560 “super-strong” promoter.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 2
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Categories
Parameters
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