Regulatory

Part:BBa_K1968001

Designed by: Paolo Marangio   Group: iGEM16_Edinburgh_OG   (2016-10-06)
Revision as of 11:51, 9 October 2016 by Marangiop (Talk | contribs)


Synechocystis Pcpc560 Phytobrick promoter

Pcpc560 stands for the 560bp region found upstream of the cpcB gene encoding the beta subunit of c-phycocyanin in Synechocystis sp. strain PCC 6803. This region has been reported to act as a promoter that is able of producing heterologous proteins at a level up to 15% of total soluble protein in Synechocystis, which is comparable to levels achieved in E. coli (Zhou et al., 2014). In light of this, this genomic region has been termed as the Pcpc560 “super-strong” promoter.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2


[edit]
Categories
Parameters
None