Composite
Part:BBa_K2066030
Designed by: Kalen Clifton, Christine Gao, Andrew Halleran, Ethan Jones, Likhitha Kolla, Joseph Maniaci, John Marken, John Mitchell, Callan Monette, Adam Reiss Group: iGEM16_William_and_Mary (2016-10-06)
Revision as of 03:50, 9 October 2016 by KalenClifton (Talk | contribs)
pTAC Ribozyme Characterization Part – GFP only; no RiboJ
This part was designed to be assembled via Gibson Assembly from WM16_014 (using primers WM16_P027 and WM16_P009) and the same WM16_014 again (using primers WM16_P019 and WM16_P025).
This part follows Lou et al. 2012 (“Ribozyme-based insulator parts buffer synthetic circuits from genetic context”). This part will be used in concert with WM16_031 (pTAC Ribozyme Chracterization Part – cI GFP; no RiboJ) to determine the influence of the RiboJ insulator sequence on gene expression.
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