Composite
ExpSubE

Part:BBa_K2020002

Designed by: Nicola Freyer, Lea Steinbeck, Svenja Meyer, Alexander Deitert   Group: iGEM16_Aachen   (2016-10-05)
Revision as of 20:21, 8 October 2016 by NicolaFreyer (Talk | contribs)


expression system for subtilisin E in E. coli

Once introduced into Escherichia coli, this BioBrick is able to produce subtilisin E and simultaneously secret the enzyme into the periplasm of the cell.


Usage and Biology

Subtilisin E is an alkaline serine protease which non-specifically digests proteins. It is naturally produced by Bacillus subtilis.
This composite part consists of the promoter BBa_R0010, the ribosome binding site BBa_B0034, the newly created BioBrick part BBa_K2020001 and the terminator BBa_B0010. BioBrick BBa_K2020001 is a composite part itself and includes the secretion tag pelB (BBa_J32015) and a subtilisin E gene optimized for E. coli codon usage (BBa_K2020000).


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 280
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//cds/enzyme/protease
//chassis/prokaryote/ecoli
Parameters
biologyBacillus subtilis
chassisEscherichia coli
proteinsubtilisin E