Primer
VF2

Part:BBa_G00100

Designed by: Tom Knight   Group: Antiquity   (2004-05-25)
Revision as of 21:44, 10 June 2007 by Smelissali (Talk | contribs)

Forward primer for sequencing/amplifying BioBrick parts (VF2)

Originally named VF2, primer binds upstream of the standard BBa_MCS on biobrick vectors.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//DNA/primerbindingsite
//plasmid/component/primerbindingsite
//primer/part/amplification
//primer/part/sequencing
Parameters
directionF
len_endn/a
len_fr314
len_start139
pct_gc50
targetBioBrick plasmid backbones
t_m50.6