Composite

Part:BBa_K2066033

Designed by: Kalen Clifton, Christine Gao, Andrew Halleran, Ethan Jones, Likhitha Kolla, Joseph Maniaci, John Marken, John Mitchell, Callan Monette, Adam Reiss   Group: iGEM16_William_and_Mary   (2016-10-07)
Revision as of 04:36, 8 October 2016 by Registry (Talk | contribs)

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pBad - RFP on UNS

This part is used to test the induction of the pBad promoter. Its insert is the I0050 functional RFP cassette from the 2016 Distribution Kit, K577882, but flanked by UNS2 and UNS3.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1287
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1227
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1019
    Illegal AgeI site found at 1873
    Illegal AgeI site found at 1985
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 1001


[edit]
Categories
Parameters
None