Composite

Part:BBa_K1890011

Designed by: Lycka Kamoen, Maria Vazquez   Group: iGEM16_TU_Delft   (2016-10-07)
Revision as of 15:44, 7 October 2016 by Registry (Talk | contribs)

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mVenus with strong consitutive promoter and RBS

mVenus is a variant of yellow fluorescent proteins (YFP) which contains several mutations that make it more stable1 and provide an improved speed and efficiency of maturation. We expressed it under the control of the strong constitutive promoter J23100 and RBS B0034, both sequences were available in the iGEM registry.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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