Regulatory

Part:BBa_K1968001:Design

Designed by: Paolo Marangio   Group: iGEM16_Edinburgh_OG   (2016-10-06)
Revision as of 21:58, 6 October 2016 by Registry (Talk | contribs)

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Synechocystis Pcpc560 Phytobrick promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2


Design Notes

Nucleotide X at position X was mutated to a X in order to remove a BsaI site, which would have interfered with later assemblies.


Source

The part was ordered as gblock through IDT and was subsequently cloned into PhytoBricks Universal Acceptor plasmids (BBa_P10500).

References