Composite

Part:BBa_K1949052:Design

Designed by: Yoshio Takata   Group: iGEM16_Tokyo_Tech   (2016-10-06)
Revision as of 12:12, 6 October 2016 by Registry (Talk | contribs)

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PBAD-rbs-amiE


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961


Design Notes

We applied a site-specific mutation to the amiE in order to destroy a recognition site of EcoRI. We changed the 291th base A to G.



Source

AmiE is derived from artificially synthesized DNA.


References