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Translational_Unit
Part:BBa_K1949051:Design
Designed by: Yoshio Takata Group: iGEM16_Tokyo_Tech (2016-10-06)
rbs-amiE
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
We applied a site-specific mutation to the amiE in order to destroy a recognition site of EcoRI. We changed the 291th base A to G.
Source
This gene was isolated from Acinetobacter sp. strain Ooi24. We artificially synthesized this.