Coding

Part:BBa_K1957003:Design

Designed by: Nancy Teng   Group: iGEM16_NRP-UEA-Norwich   (2016-10-06)
Revision as of 10:35, 6 October 2016 by Nteng (Talk | contribs) (→‎Design Notes)


HydB (N-terminally tagged) subunit of FeFe Hydrogenase


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1
    Illegal BsaI.rc site found at 369


Design Notes

This is one of of the three FeFe Hydrogenase subunits. To make the entire FeFe Hydrogenase construct the following BioBricks need to be ligated with BBa_K1957003: BBa_K1957004 and BBa_K1957001. pSB1C3 would not conjugate properly into S.oneidensis, thus the three subunits were ligated using golden gate cloning into the pBAD expression vector.

Source

The source of the part will be its sequence which was retrieved from GenBank.


References