Regulatory

Part:BBa_K2110006

Designed by: Le He   Group: iGEM16_Tianjin   (2016-10-06)
Revision as of 07:16, 6 October 2016 by Registry (Talk | contribs)

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TPA regulatory gene expression system

This part consists of the enhanced promoter PGK1(BBa_K2110012), the tpaR gene(BBa_K2110002), and the terminator CYC1（BBa_K2110000）. The tpaR gene encodes the terephthalic acid (TPA) positive feedback regulatory protein. This protein can bind the TPA inducible leader sequence (TILS) at the existence of TPA and induce the expression of downstream gene. The tpaR protein belongs to the IclR protein family and has helix-turn-helix domain.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1581
    Illegal AgeI site found at 1259
    Illegal AgeI site found at 1453
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//regulation/positive
Parameters
chassissaccharomyces cerevisiae