Regulatory

Part:BBa_K2110005

Designed by: Le He   Group: iGEM16_Tianjin   (2016-10-06)
Revision as of 06:59, 6 October 2016 by Registry (Talk | contribs)

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TPA inducible RFP expression system

PETase gene encodes the PETase enzyme which can degrade PET to terephthalic acid (TPA) and ethylene glycol (EG) with another enzyme MHETase. However, the product TPA can cause inhibition of the reaction and slow down it. This expression system consists of the TPA inducible Leader sequence, the enhanced promoter PGK1(BBa_K2110012), the PETase gene(BBa_K2110800) and Terminator CYC1(BBa_K2110000). It can be induced by product TPA so that this is a positive feedback system. This system is used in Saccharomyces cerevisiae.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1446
    Illegal AgeI site found at 1558
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//regulation/positive
Parameters
chassissaccharomyces cerevisiae