Coding

Part:BBa_K1959002

Designed by: Junyu Chen   Group: iGEM16_SCAU-China   (2016-10-04)
Revision as of 03:36, 6 October 2016 by Chenjunyu (Talk | contribs)


Modification of β-carotene hydroxylase (BHY)

This part codes for beta-carotene hydroxylase(BHY), which is needed for astaxanthin biosynthesis. The BHY cds is fused with Pea transpsit peptide to help BHY folding correctly in Orazy ssativa cell. BHY catalyzes the convertion of beta-carotene to zeaxanthin. The part is original from Haematococcus pluvialis and codon optimization has been made in order to efficiently express in Orazy sativa.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 334
    Illegal NgoMIV site found at 726
    Illegal NgoMIV site found at 793
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None