Coding

Part:BBa_K1975005

Designed by: Joachim Steen Larsen   Group: iGEM16_UNIK_Copenhagen   (2016-10-05)
Revision as of 00:33, 6 October 2016 by Registry (Talk | contribs)

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Cyan Fluorescent Protein

This is Cyan Fluorescent Protein that can be used to tag proteins of interest. It is a fluorescent protein that will show a blue color when it is excited at 405nm and it has the highest absorbance at 485nm. This sequence has been used to tag PhaA to visualize the expression of the protein. If the protein was expressed correctly, the cells would show a blue color when excited. The sequence is optimized for use in Bacillus subtilis.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 694
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None