Part:BBa_K2020002:Design

expression system for subtilisin E in E. coli

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 280
  
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

The sequence of the subtilisin E gene from Bacillus subtilis was codon optimized for E. coli with the DNA and protein sequence analysis software "Geneious" and additionally with the "Codon Optimization Tool" from IDT.

Source

The sequences of the BioBrick parts were obtained through the Registry.

• LacI promoter BBa_R0010
• RBS BBa_B0034
• leader sequence pelB (BBa_J32015) + subtilisin E gene ( BBa_K2020000)
• terminator BBa_B0010

The construct was ordered at IDT.